Two-dimensional capillary electrophoresis: Capillary isoelectric focusing and capillary zone electrophoresis with laser-induced fluorescence detection
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چکیده
منابع مشابه
Two-dimensional capillary electrophoresis: capillary isoelectric focusing and capillary zone electrophoresis with laser-induced fluorescence detection.
CIEF and CZE are coupled with LIF detection to create an ultrasensitive 2-D separation method for proteins. In this method, two capillaries are joined through a buffer-filled interface. Separate power supplies control the potential at the injection end of the first capillary and at the interface; the detector is held at ground potential. Proteins are labeled with the fluorogenic reagent Chromeo...
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Capillary electrophoresis (CE) enables rapid separations with high separation efficiency and compatibility with small sample volumes. Laser-induced fluorescence detection can result in extremely low limits of detection in CE. Single-channel fluorescence detection, however, furnishes little qualitative information about a species being detected, except for its CE migration time. Use of multidime...
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ژورنال
عنوان ژورنال: ELECTROPHORESIS
سال: 2010
ISSN: 0173-0835,1522-2683
DOI: 10.1002/elps.201000151